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Anti-inflammatory effect of hASC-EVs on IL-1β stimulated human <t>chondrocytes</t> (HC-OA). A Confocal images of HC-OAs treated with PKH67-labeled hASC-EVs, showing intracellular uptake. Scale bar, 20 μm. B – F The mRNA expression of TRPA1, COX-2, MMP-2, MMP-3, and MMP-9 was quantified using qPCR and normalized to GAPDH. Data are presented as means ± SD ( n = 3). ns, not significant ( p > 0.05), * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. IL-1β only group
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Anti-inflammatory effect of hASC-EVs on IL-1β stimulated human <t>chondrocytes</t> (HC-OA). A Confocal images of HC-OAs treated with PKH67-labeled hASC-EVs, showing intracellular uptake. Scale bar, 20 μm. B – F The mRNA expression of TRPA1, COX-2, MMP-2, MMP-3, and MMP-9 was quantified using qPCR and normalized to GAPDH. Data are presented as means ± SD ( n = 3). ns, not significant ( p > 0.05), * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. IL-1β only group
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Anti-inflammatory effect of hASC-EVs on IL-1β stimulated human <t>chondrocytes</t> (HC-OA). A Confocal images of HC-OAs treated with PKH67-labeled hASC-EVs, showing intracellular uptake. Scale bar, 20 μm. B – F The mRNA expression of TRPA1, COX-2, MMP-2, MMP-3, and MMP-9 was quantified using qPCR and normalized to GAPDH. Data are presented as means ± SD ( n = 3). ns, not significant ( p > 0.05), * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. IL-1β only group
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Anti-inflammatory effect of hASC-EVs on IL-1β stimulated human <t>chondrocytes</t> (HC-OA). A Confocal images of HC-OAs treated with PKH67-labeled hASC-EVs, showing intracellular uptake. Scale bar, 20 μm. B – F The mRNA expression of TRPA1, COX-2, MMP-2, MMP-3, and MMP-9 was quantified using qPCR and normalized to GAPDH. Data are presented as means ± SD ( n = 3). ns, not significant ( p > 0.05), * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. IL-1β only group
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Anti-inflammatory effect of hASC-EVs on IL-1β stimulated human <t>chondrocytes</t> (HC-OA). A Confocal images of HC-OAs treated with PKH67-labeled hASC-EVs, showing intracellular uptake. Scale bar, 20 μm. B – F The mRNA expression of TRPA1, COX-2, MMP-2, MMP-3, and MMP-9 was quantified using qPCR and normalized to GAPDH. Data are presented as means ± SD ( n = 3). ns, not significant ( p > 0.05), * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. IL-1β only group
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Anti-inflammatory effect of hASC-EVs on IL-1β stimulated human <t>chondrocytes</t> (HC-OA). A Confocal images of HC-OAs treated with PKH67-labeled hASC-EVs, showing intracellular uptake. Scale bar, 20 μm. B – F The mRNA expression of TRPA1, COX-2, MMP-2, MMP-3, and MMP-9 was quantified using qPCR and normalized to GAPDH. Data are presented as means ± SD ( n = 3). ns, not significant ( p > 0.05), * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. IL-1β only group
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Anti-inflammatory effect of hASC-EVs on IL-1β stimulated human chondrocytes (HC-OA). A Confocal images of HC-OAs treated with PKH67-labeled hASC-EVs, showing intracellular uptake. Scale bar, 20 μm. B – F The mRNA expression of TRPA1, COX-2, MMP-2, MMP-3, and MMP-9 was quantified using qPCR and normalized to GAPDH. Data are presented as means ± SD ( n = 3). ns, not significant ( p > 0.05), * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. IL-1β only group

Journal: Stem Cell Research & Therapy

Article Title: Extracellular vesicles from human adipose-derived stem cells relieve pain and inflammation in a rat model of knee osteoarthritis

doi: 10.1186/s13287-026-04932-7

Figure Lengend Snippet: Anti-inflammatory effect of hASC-EVs on IL-1β stimulated human chondrocytes (HC-OA). A Confocal images of HC-OAs treated with PKH67-labeled hASC-EVs, showing intracellular uptake. Scale bar, 20 μm. B – F The mRNA expression of TRPA1, COX-2, MMP-2, MMP-3, and MMP-9 was quantified using qPCR and normalized to GAPDH. Data are presented as means ± SD ( n = 3). ns, not significant ( p > 0.05), * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. IL-1β only group

Article Snippet: Human OA chondrocytes (HC-OA; Cell Applications, USA) were derived from human articular cartilage obtained from donors diagnosed with osteoarthritis, as defined by the vendor.

Techniques: Labeling, Expressing